Background: Puberty is a pivotal stage in female animal development, and marks the onset of reproductive capability.\nHowever, little is known about the function of lncRNAs (long noncoding RNAs) in puberty. Therefore, RNA-seq analysis\nwere performed between goats and rats to clarify the roles of lncRNAs and mRNAs in the onset of puberty.\nResults: In the present study, the length of lncRNAs, the length of the open reading frame and the exon count were\ncompared between the two species. Furthermore, functional annotation analysis based on Kyoto Encyclopedia of\nGenes and Genomes (KEGG) and Gene Ontology (GO) analysis of lncRNAs target genes and differentially expressed\nmRNA demonstrated the significantly enriched terms, such as AMPK signaling pathway, oxytocin signaling pathway,\ninsulin secretion as well as pheromone receptor activity, and some other signaling pathways which were involved in\nthe regulation of female puberty. Moreover, our results of siRNA interference in vitro showed the candidate lncRNA\nXLOC_446331 may play a crucial role in regulating female puberty.\nConclusion: In conclusion, the RNA-seq analysis between goat and rat provide novel candidate regulators for genetic\nand molecular studies on female puberty.
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